DNA is a widely available commercial diagnostic for the oil and gas industry, with its origins in monitoring microbially influenced corrosion (MIC) or H2S development via sulfate reducing bacteria (SRB). In practice, microbes are readily extractable from produced fluid samples owing to the high concentration of microbes (high biomass). However, the use of tracking DNA with well cuttings and cores is substantially more difficult due to drilling muds and the low concentration of microbes (low biomass) in those samples.
Total fluid movement of combined oil and water
Non-disruptive to well operations; no shutdown time, lost production or deployment of donwhole tools
Environmentally safe and friendly; low carbon footprint
Long-term monitoring with no sample storage
Unique fluid profiling by characterizing well cuttings and produced fluid
Field level analysis with 2 week turnaround on fluid results
Analysis integrated with type curves, logs, completion designs and operational events
A fraction of the cost of other diagnostics and affordable long term monitoring
Biota’s proprietary Standard Operating Procedures (SOP’s) to capture cuttings and fluid samples reflect the extensive experience of having deployed the technology on 1,300+ wells. While cold storage and shipping of samples is commonly used, advanced sample stabilization methods are available for sample preservation.
Low biomass cuttings samples can harbor microbial biomass on the order of 50-100 genomes, and reagent microbes can dominate the downstream DNA marker sequences. The sequencing of negative extraction controls (NEC) allows for the identification of microbes that have metabolisms incompatible with the extreme subsurface environments. These reagent microbes can be dropped from samples computationally before performing downstream analysis.