Biology Data Business

Technical Foundation

Microbes characterize the subsurface and fluid movement

DNA Subsurface Signature

Microbes present in the subsurface exploit local changes in physical and chemical conditions and therefore are highly associated with the local subsurface areas. The differences in microbial communities are seen from the DNA sequences being analyzed via bioinformatics.

We look at the presence/absence, abundance, diversity, and evolutionary relationships, which are used to create a database of DNA marker sequences that become highly associated with different spatial regions throughout the vertical strata, formations and along laterals.

Subsurface Samples
Microbial Community
DNA Extraction & Sequencing
DNA Diagnostics

Subsurface DNA is Ubiquitous and Resilient

Microbes exist in the subsurface pore space, even if that is at the micrometer scale, which is typically seen in oil and gas reservoirs. Furthermore, they can be found in extreme environments like high temperatures and living bacteria can be found in 100M year old formations. (Science, July 2020)

"Living" bacteria isolated from 100 million year old clay (Science, July 2020)
Ocean bed cores (Science, July 2020)
Diverse, connected communities in um pore space in a Pyrite Mine - scale bar 10 μm (Nature, Scientific Reports, 2018)

From DNA to Diagnostic

Stanford Research at Sanford Underground Research Facility, SD (2019), used technology co-developed by Biota’s technology team. They analyzed formation water samples from new boreholes near mine shafts and used microbial fluid signatures to ID well connectivity from natural fractures. The hypothesis supported by analysis of coring run intersecting wells.

Indigenous microbial community composition in reservoir‐pro-duced fluids may be used as unique signatures to identify natural interwell connectivity or track the origin of fluids.